Spatial Configuration of Transposable Element Ac Termini Affects Their Ability to Induce Chromosomal Breakage in Maize
نویسندگان
چکیده
منابع مشابه
Spatial configuration of transposable element Ac termini affects their ability to induce chromosomal breakage in maize.
Composite or closely linked maize (Zea mays) Ac/Ds transposable elements can induce chromosome breakage, but the precise configurations of Ac/Ds elements that can lead to chromosome breakage are not completely defined. Here, we determined the structures and chromosome breakage properties of 15 maize p1 alleles: each allele contains a fixed fractured Ac (fAc) element and a closely linked full-le...
متن کاملA hyperactive transposase of the maize transposable element activator (Ac).
Activator/Dissociation (Ac/Ds) transposable elements from maize are widely used as insertional mutagenesis and gene isolation tools in plants and more recently also in medaka and zebrafish. They are particularly valuable for plant species that are transformation-recalcitrant and have long generation cycles or large genomes with low gene densities. Ac/Ds transposition frequencies vary widely, ho...
متن کاملCharacterization of the maize transposable element Ac by internal deletions.
We have used the ability of Ac to transpose in tobacco to determine which Ac sequences are required for transposition, using a phenotypic assay for Ac excision from an NPTII gene in which excisions of Ac result in calli resistant to the antibiotic kanamycin (Baker et al., 1987). Here we show that deletion of the Ac DNA which encodes the untranslated leader of the Ac transcript does not prevent ...
متن کاملChromosome breakage by pairs of closely linked transposable elements of the Ac-Ds family in maize.
Chromosome breaks and hence chromosomal rearrangements often occur in maize stocks harboring transposable elements (TEs), yet it is not clear what types of TE structures promote breakage. We have shown previously that chromosomes containing a complex transposon structure consisting of an Ac (Activator) element closely linked in direct orientation to a terminally deleted or fractured Ac (fAc) el...
متن کاملMutational analysis of the N terminus of the protein of maize transposable element Ac.
Mutations of transposable element Ac were tested for their capability to excise themselves from their location autonomously, to be excised by an active Ac, or to act in trans in the excision of an Ac delta element. Removal of 101 amino acids from the N terminus of the Ac protein does not decrease excision. A cis-acting site between base pairs 186 and 207 is important for excision by the wild-ty...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 2010
ISSN: 1532-298X,1040-4651
DOI: 10.1105/tpc.109.070052